Role of Royal Jelly Treated Adipose-Derived Stem Cell-Extracellular Vesicles on Fibroblast Proliferation, Migration, and Collagen Production

Extracellular vesicles (EVs) secreted from adipose-derived stem cells (ADSCs) are known to exhibit collagen synthesis and migration activity in fibroblasts. These EVs can be used as a regenerative medicine for cosmetic medicinal materials. Royal jelly (RJ) is produced the hypopharyngeal mandibular glands of honeybees (nurse bee), which contains apicin 10-hydroxy-2-decenoic acid, compounds that promote fibroblast proliferation elastin synthesis. Here, we investigated whether RJ could further enhance physiological function ADSC-EVs on The findings confirmed lyophilized enzyme-treated enhanced secretion by ADSCs, increasing proliferation, migration, fibroblasts compared those induced conventional ADSC-EVs. Bioinformatics analysis was conducted using various software programs, based data obtained comprehensive gene expression small RNA profiling analyses through next-generation sequencing; results suggested treatment significantly genes related extracellular matrix composition. Our suggest qualitatively altered offer more effective therapeutic material than ADSCs-EVs.